Regulatory

Part:BBa_K4721006:Design

Designed by: Celeste Bos   Group: iGEM23_Leiden   (2023-10-11)


PL/O4/A1 IPTG inducible system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 90
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The basic part for the lacIq promoter also contains a spacer sequence, after which the PL/O4/A1 part was placed

Source

This composite part is comprised of four basic parts, the lacIq repressor gene and its terminator, which is expressed by the lacIq promoter, and the PL/O4/A1, containing the lacO operator sequence. The part was designed by Carrillo et al. (2019)[1]

References

[1] Carrillo M, Wagner M, Petit F, Dransfeld A, Becker A, Erb TJ. Design and Control of Extrachromosomal Elements in Methylorubrum extorquens AM1. ACS Synth Biol. 2019;8(11):2451-2456. doi:10.1021/acssynbio.9b00220

[2] Schada Von Borzyskowski L, Remus-Emsermann M, Weishaupt R, Vorholt JA, Erb TJ. A set of versatile brick vectors and promoters for the assembly, expression, and integration of synthetic operons in methylobacterium extorquens am1 and other alphaproteobacteria. ACS Synth Biol. 2015;4(4):430-443. doi:10.1021/sb500221v

[3] Peyraud R, Kiefer P, Christen P, Massou S, Portais JC, Vorholt JA. Demonstration of the ethylmalonyl-CoA pathway by using13C metabolomics. Proc Natl Acad Sci U S A. 2009;106(12):4846-4851. doi:10.1073/pnas.0810932106

[4] Leveau JHJ, Lindow SE. Predictive and Interpretive Simulation of Green Fluorescent Protein Expression in Reporter Bacteria. J Bacteriol. 2001;183(23):6752-6762. doi:10.1128/JB.183.23.6752-6762.2001